Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.040485 |
Chromosome: | scaffold 36 |
Location: | 9406 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre36.g759647 | (1 of 4) 3.1.3.24 - Sucrose-phosphate phosphatase / Sucrose-6-phosphate phosphatase | intron |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACATTGCGTGGTAAATGGTTGTGATTAATA |
Internal bar code: | GAGTTACGGCGGTGCGAGTCAG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 504 |
LEAP-Seq percent confirming: | 99.3911 |
LEAP-Seq n confirming: | 2122 |
LEAP-Seq n nonconfirming: | 13 |
LEAP-Seq n unique pos: | 21 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CATTTGTCGTTGGTCAGGTG |
Suggested primer 2: | ATAGGGAGAATGGGGTACGG |