| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.040507 |
| Chromosome: | chromosome 5 |
| Location: | 2522779 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| intergenic |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTACAATTCTAAGTCTCCAGTGCTCAACAT |
| Internal bar code: | GCTGCTACTGCGAGGCAGTTGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 710 |
| LEAP-Seq percent confirming: | 97.4101 |
| LEAP-Seq n confirming: | 22304 |
| LEAP-Seq n nonconfirming: | 593 |
| LEAP-Seq n unique pos: | 129 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTATCCAACTGCGACCGATT |
| Suggested primer 2: | GTTACTGAGTCCTCTGCCGC |