| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.040512 |
| Chromosome: | chromosome 6 |
| Location: | 2683713 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g270276 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACCATCTGTGCATGGGGTCGTGCATGGGGC |
| Internal bar code: | TGAGTCGATCCATCTTATGTCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 686 |
| LEAP-Seq percent confirming: | 98.9506 |
| LEAP-Seq n confirming: | 3206 |
| LEAP-Seq n nonconfirming: | 34 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGAACACCACGTTCCACCT |
| Suggested primer 2: | ATGCAGACAGTGAGGCTGTG |