Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.040542 |
Chromosome: | chromosome_3 |
Location: | 4792044 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre03.g178500 | RIB43,RIB43A | Flagellar protofilament ribbon protein | sense | intron |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | TTACCTGCTCAGATCCAGCAGCTCCCGCCT |
Internal bar code: | CAACAAATGGGCTGCGTCTATA |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 892 |
LEAP-Seq percent confirming: | 98.9514 |
LEAP-Seq n confirming: | 23968 |
LEAP-Seq n nonconfirming: | 254 |
LEAP-Seq n unique pos: | 88 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | ATGCCATGTACGTGCTGTGT |
Suggested primer 2: | CCTCTCCGTCAAACTTCTGC |