| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.040590 |
| Chromosome: | chromosome 16 |
| Location: | 1533048 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g653338 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAGCGAGGTCGCCGCCGATGGTGATGCAGC |
| Internal bar code: | TCGAACCCATGTGAGTGCTCCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 727 |
| LEAP-Seq percent confirming: | 85.2962 |
| LEAP-Seq n confirming: | 2361 |
| LEAP-Seq n nonconfirming: | 407 |
| LEAP-Seq n unique pos: | 23 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCAGCACTACAGTCAGCACC |
| Suggested primer 2: | AACGCATTGTGTTGGACAAA |