| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.040598 |
| Chromosome: | chromosome 17 |
| Location: | 2146511 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g712771 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCGGTAACCGCCCCCATACCGATCGGTTTA |
| Internal bar code: | GACACTGACAGGATCTGTTCCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 980 |
| LEAP-Seq percent confirming: | 97.8261 |
| LEAP-Seq n confirming: | 360 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGCATTCCTCATTCCTCGT |
| Suggested primer 2: | TATGGGGGTTGGTGAGGTAA |