Insertion junction: LMJ.RY0402.040601_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.040601
Chromosome:chromosome 10
Location:2438766
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre10.g435800 CSP41b,CSP41B,SNE11,RAP38 Chloroplast stem-loop-binding protein 41b; (1 of 1) PTHR10366:SF289 - CHLOROPLAST STEM-LOOP BINDING PROTEIN OF 41 KDA B, CHLOROPLASTIC intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCAAATTAGGCCCTGCCACCCCGCCCTTGG
Internal bar code:GGCTGCCGCCCCAGCCCTGGAC

Confirmation - LEAP-Seq

LEAP-Seq distance:225
LEAP-Seq percent confirming:86.2796
LEAP-Seq n confirming:3314
LEAP-Seq n nonconfirming:527
LEAP-Seq n unique pos:14

Suggested primers for confirmation by PCR

Suggested primer 1:CACCCTCACACATTCACGAC
Suggested primer 2:AATCCACTACAACGCCAAGG