| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.040618 |
| Chromosome: | chromosome_2 |
| Location: | 2809015 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre02.g094450 | PHC21 | Cell wall protein pherophorin-C21 | sense | 5'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | GTCTAGACCCAGCGAGGCGCACGTCCCCAT |
| Internal bar code: | AAGTAGGTGGGCACGCAGGTAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 740 |
| LEAP-Seq percent confirming: | 99.2022 |
| LEAP-Seq n confirming: | 9574 |
| LEAP-Seq n nonconfirming: | 77 |
| LEAP-Seq n unique pos: | 42 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAGGGCTATGTGTACAGCCG |
| Suggested primer 2: | AGGTTAAGGCGGGTAGCAAT |