| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.040627 |
| Chromosome: | chromosome_8 |
| Location: | 4620648 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre08.g383400 | antisense | 5'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | CCGAACTGGTGGTCTAGGCAAGGGTAGCCT |
| Internal bar code: | TGTTAGGTGTGTACTGTTCTCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 604 |
| LEAP-Seq percent confirming: | 97.9427 |
| LEAP-Seq n confirming: | 4475 |
| LEAP-Seq n nonconfirming: | 94 |
| LEAP-Seq n unique pos: | 18 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGATGTCAGCTACCAGCACC |
| Suggested primer 2: | TTCGTGGGGGTTGATATTGT |