| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.040634 |
| Chromosome: | chromosome 3 |
| Location: | 7955601 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g202100 | OPR18 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGCTAGGCGCTGTGCCAGGCCGTCTCCATT |
| Internal bar code: | CATCCACCTATTCGTCCCGCTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 924 |
| LEAP-Seq percent confirming: | 99.612 |
| LEAP-Seq n confirming: | 12323 |
| LEAP-Seq n nonconfirming: | 48 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATACCTCTCCCACGTTGTGC |
| Suggested primer 2: | ATGAGGCTGCTGTTACGCTT |