| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.040665 |
| Chromosome: | chromosome 12 |
| Location: | 2509061 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g506400 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTAGACTTAGAGCCTCACCATTTCCGGCAA |
| Internal bar code: | CCACGGACAGCGCGACCGTGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 397 |
| LEAP-Seq percent confirming: | 91.6509 |
| LEAP-Seq n confirming: | 483 |
| LEAP-Seq n nonconfirming: | 44 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGGTCTCTCACGTGCCTTC |
| Suggested primer 2: | TACCTCCATTACGCACTCCC |