| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.040682 |
| Chromosome: | chromosome_2 |
| Location: | 1086970 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre02.g081050 | FAP24 | Flagellar Associated Protein | antisense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | CGACGGGACATCCCAAACTACGCAAAATCC |
| Internal bar code: | CGGGAGGTAAATAGCTAGAGTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 750 |
| LEAP-Seq percent confirming: | 99.1129 |
| LEAP-Seq n confirming: | 32177 |
| LEAP-Seq n nonconfirming: | 288 |
| LEAP-Seq n unique pos: | 20 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAACCTAGCCTCACAGCACA |
| Suggested primer 2: | TAATGCAATGCCGTGTTGTT |