| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.040684 |
| Chromosome: | chromosome 3 |
| Location: | 2966207 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g163400 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGTGGTTATGCATGCCGTGCTCGTACAGT |
| Internal bar code: | TAACGGATGTGCGGAAGTCGAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 834 |
| LEAP-Seq percent confirming: | 99.6651 |
| LEAP-Seq n confirming: | 16963 |
| LEAP-Seq n nonconfirming: | 57 |
| LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAAAGAATCATGTACGGCGG |
| Suggested primer 2: | AGGATCCCATGGGTACATGA |