| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.040688 |
| Chromosome: | chromosome 2 |
| Location: | 3612447 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g095141 | (1 of 1) IPR002938//IPR006076 - FAD-binding domain // FAD dependent oxidoreductase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCGTGGGGAGCGATGATGGGTAGGGGGCGT |
| Internal bar code: | GTGCTTTTCAGTTGATCCTCGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 600 |
| LEAP-Seq percent confirming: | 72.8439 |
| LEAP-Seq n confirming: | 1647 |
| LEAP-Seq n nonconfirming: | 614 |
| LEAP-Seq n unique pos: | 19 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTGAGCGGTAGTAGCCGAGT |
| Suggested primer 2: | GGTGTTTGTGTTGATGCTGG |