| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.040693 |
| Chromosome: | chromosome 12 |
| Location: | 5610361 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g532050 | (1 of 1) PF00069//PF04564//PF07714//PF12796 - Protein kinase domain (Pkinase) // U-box domain (U-box) // Protein tyrosine kinase (Pkinase_Tyr) // Ankyrin repeats (3 copies) (Ank_2) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGAGCAGGACTTGAGCAGAGGTCAGGGTGC |
| Internal bar code: | TGTTACGGGGGATTTGGCGAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 479 |
| LEAP-Seq percent confirming: | 98.548 |
| LEAP-Seq n confirming: | 6176 |
| LEAP-Seq n nonconfirming: | 91 |
| LEAP-Seq n unique pos: | 42 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACCCCATCACCATACCTCAA |
| Suggested primer 2: | TCTGGTGTACGAGCTGATGC |