Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.040698 |
Chromosome: | chromosome 7 |
Location: | 1957704 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
intergenic |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAGTGCCGCCAGCGGGATTGCCGGAAAGCG |
Internal bar code: | TGGAGACGGGTGGCGGAAGTCT |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 748 |
LEAP-Seq percent confirming: | 99.4078 |
LEAP-Seq n confirming: | 2518 |
LEAP-Seq n nonconfirming: | 15 |
LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GTTGGCTTTCTCACCTCTGC |
Suggested primer 2: | TATGCAATGAGCCAAAGCTG |