| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.040698 |
| Chromosome: | chromosome 7 |
| Location: | 1958233 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g325752 | FAP258 | Flagellar Associated Protein | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCGGGCGAGAGGCTTTGAGGCGATGCAGGT |
| Internal bar code: | GTTTGTATTTCATATGTCATCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 230 |
| LEAP-Seq percent confirming: | 98.8235 |
| LEAP-Seq n confirming: | 84 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGCATGTCGTGAAGCGTAG |
| Suggested primer 2: | GTACCCTCACTTGATGCCGT |