| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.040750 |
| Chromosome: | chromosome 17 |
| Location: | 3546535 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g725450 | (1 of 1) K11789 - HIV-1 Vpr-binding protein (VPRBP, DCAF1) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTACCTGGACGCGCTGGGGCCGGTGCTGGC |
| Internal bar code: | ATTGGAACCCGATCATGGGGTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 39 |
| LEAP-Seq percent confirming: | 96.5517 |
| LEAP-Seq n confirming: | 28 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TAGCTATGCGTCACTTGCCA |
| Suggested primer 2: | GAATTCCGCCATTAACAGGA |