Insertion junction: LMJ.RY0402.040762_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.040762
Chromosome:chromosome 9
Location:7521225
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre09.g414100 (1 of 33) IPR013830 - SGNH hydrolase-type esterase domain 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGGATTAAGCCGTCTAAATTTATTCTAGGA
Internal bar code:TGGTCCCTTGCAATATTTTTAC

Confirmation - LEAP-Seq

LEAP-Seq distance:0
LEAP-Seq percent confirming:0.0389408
LEAP-Seq n confirming:1
LEAP-Seq n nonconfirming:2567
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:CAACCGCCTTCTGATCTCTC
Suggested primer 2:GTCACACGGCAGAGAGTTGA