| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.040792 |
| Chromosome: | chromosome 2 |
| Location: | 4237021 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g098750 | (1 of 7) PF00027//PF00520 - Cyclic nucleotide-binding domain (cNMP_binding) // Ion transport protein (Ion_trans) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGCCCCGCCGACCCGCTGATCACCCAGTAC |
| Internal bar code: | GAATGAAAGGAGCTGTGTAGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 575 |
| LEAP-Seq percent confirming: | 66.1205 |
| LEAP-Seq n confirming: | 1251 |
| LEAP-Seq n nonconfirming: | 641 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACACACACACACACACACCG |
| Suggested primer 2: | ACTTTGCGTTTCCACTGCTT |