| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.040857 |
| Chromosome: | chromosome 16 |
| Location: | 633955 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g691950 | (1 of 6) IPR006502//IPR011333 - Protein of unknown function PDDEXK-like // POZ domain | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACACACGTTGCGTCGGGCCTGACCGATTTA |
| Internal bar code: | GTCACGCCATAAGTTTCAGTGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 738 |
| LEAP-Seq percent confirming: | 98.6161 |
| LEAP-Seq n confirming: | 2494 |
| LEAP-Seq n nonconfirming: | 35 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCTGCCCTGCTAACTCCACT |
| Suggested primer 2: | TTCTACTGCTGCATCAACGG |