| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.040874 |
| Chromosome: | chromosome 9 |
| Location: | 2406528 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g391600 | MEP1 | Putative IgA-specific metalloendopeptidase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGTACAAGAGTGGACGCATGTCGGTACGCC |
| Internal bar code: | CGCACCCGCTTGTGGCTCTGCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 756 |
| LEAP-Seq percent confirming: | 98.7244 |
| LEAP-Seq n confirming: | 4102 |
| LEAP-Seq n nonconfirming: | 53 |
| LEAP-Seq n unique pos: | 27 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGTTCTATAAGCGCCAGCA |
| Suggested primer 2: | AGGCCCTTGACCGAGTAGAT |