Insertion junction: LMJ.RY0402.040941_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.040941
Chromosome:chromosome 1
Location:2172760
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre01.g011850 (1 of 8) 2.7.11.1//2.7.12.1 - Non-specific serine/threonine protein kinase / Threonine-specific protein kinase // Dual-specificity kinase 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTGTCAGGTTTCACATCACCAAACAAATGC
Internal bar code:TGCGTACATGTCAGTGCCATCA

Confirmation - LEAP-Seq

LEAP-Seq distance:1012
LEAP-Seq percent confirming:99.275
LEAP-Seq n confirming:6710
LEAP-Seq n nonconfirming:49
LEAP-Seq n unique pos:51

Suggested primers for confirmation by PCR

Suggested primer 1:GAGTCTGTATCCCGCTCTGC
Suggested primer 2:CTGACCGTATGTATGGCACG