| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.040978 |
| Chromosome: | chromosome 6 |
| Location: | 4212250 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g278236 | UMM12 | (1 of 1) PTHR10108:SF871 - METHYLTRANSFERASE; Putative ubiquinone / menaquinone biosynthesis methyltransferase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCCAGCTCCTGCGCTCAGGGTGTGTGCAAG |
| Internal bar code: | TTCTCTAGGCCAATCGAGGAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 923 |
| LEAP-Seq percent confirming: | 99.7321 |
| LEAP-Seq n confirming: | 4840 |
| LEAP-Seq n nonconfirming: | 13 |
| LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCAGTACAAGCAGCAATGGA |
| Suggested primer 2: | GTGGAAGTAGGAGCCCACAA |