| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.040987 |
| Chromosome: | chromosome 1 |
| Location: | 2746386 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g016250 | MLO3,MLO4 | (1 of 5) PF03094 - Mlo family (Mlo); MLO family protein | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCCGCGAGACCCAAATGTGCAGCACGACG |
| Internal bar code: | CGCAAAATTATACCTTTCACAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 232 |
| LEAP-Seq percent confirming: | 96.3265 |
| LEAP-Seq n confirming: | 708 |
| LEAP-Seq n nonconfirming: | 27 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGGCCACAAGCTCTTATTGT |
| Suggested primer 2: | AACGGCTTACAGTGGGATTG |