| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.041062 |
| Chromosome: | chromosome 4 |
| Location: | 2033054 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g219000 | (1 of 2) PTHR10887//PTHR10887:SF333//PTHR13779//PTHR13779:SF20 - DNA2/NAM7 HELICASE FAMILY // SUBFAMILY NOT NAMED // HOLLIDAY JUNCTION DNA HELICASE RUVB-RELATED // SUBFAMILY NOT NAMED | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGGAGAAGGCAGCGCCACATTGCATTAGG |
| Internal bar code: | CGCCGGCGTTTCCTACAAAGCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 860 |
| LEAP-Seq percent confirming: | 99.6884 |
| LEAP-Seq n confirming: | 12157 |
| LEAP-Seq n nonconfirming: | 38 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGAAGGTACACCCATGCTG |
| Suggested primer 2: | AACGGGGAAGCAGTTCTTTT |