Insertion junction: LMJ.RY0402.041107_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.041107
Chromosome:chromosome 2
Location:4160761
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre02.g098200 CGL103,PRT1 (1 of 1) PF00569//PF13920 - Zinc finger, ZZ type (ZZ) // Zinc finger, C3HC4 type (RING finger) (zf-C3HC4_3); Conserved in the Green Lineage intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGACTGGGGAGGGTGCGTCGCCATTGCCGT
Internal bar code:GAGACGCTCACGGTCCGAACAC

Confirmation - LEAP-Seq

LEAP-Seq distance:780
LEAP-Seq percent confirming:76.7428
LEAP-Seq n confirming:2521
LEAP-Seq n nonconfirming:764
LEAP-Seq n unique pos:10

Suggested primers for confirmation by PCR

Suggested primer 1:TGAACGGAAACATTGAACCA
Suggested primer 2:AGAGCACAAGAAGAGCCAGC