| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.041111 |
| Chromosome: | chromosome 12 |
| Location: | 7796188 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g553500 | ANK15 | Predicted protein with ankyrin repeats; (1 of 78) IPR002110//IPR020683 - Ankyrin repeat // Ankyrin repeat-containing domain | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCAGTCAGCGGGTCTTTGCGGAGCCGCGG |
| Internal bar code: | CACGCGATCGACGAGCGTTTGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 794 |
| LEAP-Seq percent confirming: | 99.3854 |
| LEAP-Seq n confirming: | 1617 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGCCAAAAGACCTAAACGAG |
| Suggested primer 2: | GCAGCAGTTCCTTCTATGGC |