| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.041112 |
| Chromosome: | chromosome 17 |
| Location: | 2944743 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g719950 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATTACTCCTTTACCACCAACAGCTGAGAAC |
| Internal bar code: | GAGCCCGCTCCGGTTTCCGGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 928 |
| LEAP-Seq percent confirming: | 97.5304 |
| LEAP-Seq n confirming: | 4897 |
| LEAP-Seq n nonconfirming: | 124 |
| LEAP-Seq n unique pos: | 44 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACCCCTGACCCTCTTTACA |
| Suggested primer 2: | AAACCTCATCTGCACCGAAG |