| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.041120 |
| Chromosome: | chromosome 7 |
| Location: | 743232 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g317800 | (1 of 1) PF04091 - Exocyst complex subunit Sec15-like (Sec15) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCACCTCTACCCCTACCCCCACCTGGGTC |
| Internal bar code: | GATCACCTTCCAAGATGGACA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 134 |
| LEAP-Seq percent confirming: | 99.1304 |
| LEAP-Seq n confirming: | 114 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTAAAATGTTAGGCGCAGC |
| Suggested primer 2: | CAAATCCCGCCCACTAAGTA |