| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.041129 |
| Chromosome: | chromosome 9 |
| Location: | 2445168 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g391370 | (1 of 781) IPR000104 - Antifreeze protein, type I | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCTCATGCGGCCGACCCACATCGCCTCCAC |
| Internal bar code: | GAGTGCAAGTCCGTCATGCAGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 442 |
| LEAP-Seq percent confirming: | 99.4118 |
| LEAP-Seq n confirming: | 169 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGCCAAAGCCGAGTTAGAG |
| Suggested primer 2: | TATCGTGCACTGCTTCGTTC |