| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.041137 |
| Chromosome: | chromosome_1 |
| Location: | 1712657 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre01.g009200 | sense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | CGGGCCGGTGTCGTGCTGAGGAGCTCTGGA |
| Internal bar code: | CCCGCTCGCAAGGCACAACGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 165 |
| LEAP-Seq percent confirming: | 91.9211 |
| LEAP-Seq n confirming: | 1445 |
| LEAP-Seq n nonconfirming: | 127 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGGTGAGAGCTTGCATTCC |
| Suggested primer 2: | ACACAGTACCGTGAGAGGGG |