Insertion junction: LMJ.RY0402.041138_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.041138
Chromosome:chromosome 2
Location:3277384
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre02.g095081 (1 of 1) PF09295 - ChAPs (Chs5p-Arf1p-binding proteins) (ChAPs) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCATGAATGTAACCCCCACCTACCCCCTAC
Internal bar code:CGCGCCTGTCTTGATCATCGTC

Confirmation - LEAP-Seq

LEAP-Seq distance:256
LEAP-Seq percent confirming:99.4063
LEAP-Seq n confirming:1172
LEAP-Seq n nonconfirming:7
LEAP-Seq n unique pos:5

Suggested primers for confirmation by PCR

Suggested primer 1:GGGGAGCAGATAACGAGATG
Suggested primer 2:CGTTCTCGTTGGTTTTGGTT