| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.041201 |
| Chromosome: | chromosome 9 |
| Location: | 5658737 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g401256 | (1 of 13) PF05050 - Methyltransferase FkbM domain (Methyltransf_21) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGACATTCACATCTGCGTGCGTGCGTGTG |
| Internal bar code: | AGCAGTCGCGTAATGAAGGCCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 220 |
| LEAP-Seq percent confirming: | 98.771 |
| LEAP-Seq n confirming: | 5867 |
| LEAP-Seq n nonconfirming: | 73 |
| LEAP-Seq n unique pos: | 26 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TAGCCCAGCTCCTCCAAGTA |
| Suggested primer 2: | ACTGAAAGGGTTGTGGTTGC |