| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.041203 |
| Chromosome: | chromosome 17 |
| Location: | 2506040 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g716150 | SUMO90,SUM4 | Similar to Small ubiquitin-like modifier; (1 of 4) PF03171//PF11976 - 2OG-Fe(II) oxygenase superfamily (2OG-FeII_Oxy) // Ubiquitin-2 like Rad60 SUMO-like (Rad60-SLD) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGACAGGGTGGCTCGGAGGTGCACTTCAA |
| Internal bar code: | TATTTTCATTTGCCAACGTTAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 989 |
| LEAP-Seq percent confirming: | 99.2334 |
| LEAP-Seq n confirming: | 5566 |
| LEAP-Seq n nonconfirming: | 43 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACTCGGTCCACTCCATTACG |
| Suggested primer 2: | GCTTGAAACCGCTGAGTAGC |