| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.041221 |
| Chromosome: | chromosome 15 |
| Location: | 1225509 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre15.g641451 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCTGAGAAAGCAATCACACAAGAGAGAACC |
| Internal bar code: | CAGGTGGAGCCAATGCAAGAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 183 |
| LEAP-Seq percent confirming: | 99.5763 |
| LEAP-Seq n confirming: | 470 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGGAGAGGGAGGAGGTGAT |
| Suggested primer 2: | CCTGGCTTGCTCTGACCTAC |