| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.041229 |
| Chromosome: | chromosome 6 |
| Location: | 5078725 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g280600 | GEX1 | Gamete-expressed protein 1; (1 of 1) PTHR33538:SF2 - PROTEIN GAMETE EXPRESSED 1 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCTTACCGCTCCGGACGGCGGCCGAGTGAC |
| Internal bar code: | CAAAGGGAGCCCGTCGGCACTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1053 |
| LEAP-Seq percent confirming: | 98.8484 |
| LEAP-Seq n confirming: | 2575 |
| LEAP-Seq n nonconfirming: | 30 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCGCATATAACGTAGGCCAT |
| Suggested primer 2: | CTACCGCCCTGACACAAACT |