Insertion junction: LMJ.RY0402.041256_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.041256
Chromosome:chromosome 16
Location:2355060
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre16.g659750 CEP15 Cysteine endopeptidase; (1 of 3) 3.4.18.1 - Cathepsin X / Lysosomal carboxypeptidase B intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GACTGGTGCGCGCGGTAGTGCAAGGGCCAT
Internal bar code:TCAACGGAAGGGTCCATGCCAC

Confirmation - LEAP-Seq

LEAP-Seq distance:878
LEAP-Seq percent confirming:96.6991
LEAP-Seq n confirming:6064
LEAP-Seq n nonconfirming:207
LEAP-Seq n unique pos:17

Suggested primers for confirmation by PCR

Suggested primer 1:AATGTTGCACTCAACCACCA
Suggested primer 2:TTTTTCGCCAATTACGGAAC