| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.041270 |
| Chromosome: | chromosome 2 |
| Location: | 2252566 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g090100 | (1 of 1) PTHR22883//PTHR22883:SF23 - ZINC FINGER DHHC DOMAIN CONTAINING PROTEIN // PALMITOYLTRANSFERASE ZDHHC4-RELATED | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTCTCCTGCTCTAGGTACACCTCCGGAGC |
| Internal bar code: | AGCCGTCGTTTATGTTAGGTCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 864 |
| LEAP-Seq percent confirming: | 99.334 |
| LEAP-Seq n confirming: | 3132 |
| LEAP-Seq n nonconfirming: | 21 |
| LEAP-Seq n unique pos: | 29 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGGTTGATGTTTGCGTGAC |
| Suggested primer 2: | AAATCAAACCAAACCAAGCG |