| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.041282 |
| Chromosome: | chromosome 12 |
| Location: | 7419612 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g556700 | (1 of 3) IPR000104//IPR011009 - Antifreeze protein, type I // Protein kinase-like domain | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGTGGGCGAAACAGCGAGCGGAGCGCGCG |
| Internal bar code: | TAGAGTGCCCAAGGAGGCGGAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 783 |
| LEAP-Seq percent confirming: | 99.5775 |
| LEAP-Seq n confirming: | 4007 |
| LEAP-Seq n nonconfirming: | 17 |
| LEAP-Seq n unique pos: | 31 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGTGCCAGTCACCTGTACCT |
| Suggested primer 2: | GTTTGCGCCTAGAACAGAGG |