| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.041285 |
| Chromosome: | chromosome 12 |
| Location: | 3779961 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g515250 | ROC7 | Rythm Of Chloroplast bioluminescence 7; (1 of 1) PF09758 - Uncharacterised conserved protein (FPL) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGAAAACCAGATTACCGACGTGTCCGTGT |
| Internal bar code: | CCGCGGCCATGGGCCGCCTAAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 655 |
| LEAP-Seq percent confirming: | 99.3344 |
| LEAP-Seq n confirming: | 1194 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACTGCTCCACTGCACAACAC |
| Suggested primer 2: | TGCTAGGCTGGACCTTCAGT |