| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.041295 |
| Chromosome: | chromosome 6 |
| Location: | 7686639 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g301802 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGAGGAAAAGGGGTTCAAGGGCCCACCCCA |
| Internal bar code: | ACAGGAGCAGCATCGGTGATTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 433 |
| LEAP-Seq percent confirming: | 39.8005 |
| LEAP-Seq n confirming: | 13168 |
| LEAP-Seq n nonconfirming: | 19917 |
| LEAP-Seq n unique pos: | 28 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTTTGTGTGTGGGTGTGTG |
| Suggested primer 2: | CCGCACTTCCTGTGTGAGTA |