Insertion junction: LMJ.RY0402.041341_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.041341
Chromosome:chromosome 6
Location:3223609
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre06.g276150 (1 of 274) IPR020683 - Ankyrin repeat-containing domain CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AGGTCTCGCCACGTTGAGGCGCGCATGCAT
Internal bar code:CCACGATCCGTGTGTCTATGGG

Confirmation - LEAP-Seq

LEAP-Seq distance:141
LEAP-Seq percent confirming:26.1538
LEAP-Seq n confirming:153
LEAP-Seq n nonconfirming:432
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:TGAGTGCTTTGTCGAACAGG
Suggested primer 2:TGCTGTTTTCAGTTGCAAGG