| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.041372 |
| Chromosome: | chromosome 12 |
| Location: | 8254866 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g550300 | (1 of 1) PTHR24361//PTHR24361:SF276 - MITOGEN-ACTIVATED KINASE KINASE KINASE // PROTEIN KINASE | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCTTCCTCACCTGGAACCGGCCCTTGCGCT |
| Internal bar code: | CGACACGTCGGATGGTTCTGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 34 |
| LEAP-Seq percent confirming: | 22.4138 |
| LEAP-Seq n confirming: | 26 |
| LEAP-Seq n nonconfirming: | 90 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GATGAGGGTGAGGAGGATGA |
| Suggested primer 2: | GTTGTGGTAGTTGCGTCAGC |