| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.041379 |
| Chromosome: | chromosome 6 |
| Location: | 6742736 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g294550 | (1 of 1) IPR000104//IPR008537 - Antifreeze protein, type I // Protein of unknown function DUF819 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATCATGAATGTAACCCCGCCCCGCCACCCT |
| Internal bar code: | GCTCCATGGGCCCAATACAGCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 128 |
| LEAP-Seq percent confirming: | 26.3701 |
| LEAP-Seq n confirming: | 1073 |
| LEAP-Seq n nonconfirming: | 2996 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGCACCTCAACGTCACTGT |
| Suggested primer 2: | CAGCAGGGAGAGTTAGCTGG |