| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.041443 |
| Chromosome: | chromosome 13 |
| Location: | 4926768 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g606101 | (1 of 2) PTHR19959//PTHR19959:SF147 - KINESIN LIGHT CHAIN // SUBFAMILY NOT NAMED | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCGGCCCCTGCGGTTTCACTCTGAGGCACA |
| Internal bar code: | ACGCAGTGGCGACGCCACTAGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 521 |
| LEAP-Seq percent confirming: | 43.9745 |
| LEAP-Seq n confirming: | 759 |
| LEAP-Seq n nonconfirming: | 967 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTGTGGTGGTCTGGAAGAT |
| Suggested primer 2: | GCAGTCAAGTCCAGCAATCA |