| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.041445 |
| Chromosome: | chromosome 7 |
| Location: | 3278873 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g334350 | (1 of 3) 2.7.1.39 - Homoserine kinase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TAATCAATTGGATTCTCGATGTCTTCCCAA |
| Internal bar code: | GTCCACGGTTAGTGTGGGGAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 512 |
| LEAP-Seq percent confirming: | 99.5183 |
| LEAP-Seq n confirming: | 3925 |
| LEAP-Seq n nonconfirming: | 19 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGGTGACGTTGTTGGTTTG |
| Suggested primer 2: | CAGCTTGAGGATTAGGACGC |