| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.041459 |
| Chromosome: | chromosome 9 |
| Location: | 5850815 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g402256 | (1 of 3) 3.4.11.1 - Leucyl aminopeptidase / Peptidase S | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GACACGATCACAGGCGGTGGTCCGACGCGG |
| Internal bar code: | ATTTATAGTTAGTTCTTAGTAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 851 |
| LEAP-Seq percent confirming: | 98.6842 |
| LEAP-Seq n confirming: | 150 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAGCTGAGACGAGAGCAAGC |
| Suggested primer 2: | TCCGTGCCCTGTTATCTTTC |