| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.041471 |
| Chromosome: | chromosome 12 |
| Location: | 2895334 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g501950 | PPP39 | Phosphoprotein phosphatase 2C-related; (1 of 70) 3.1.3.16 - Protein-serine/threonine phosphatase / Serine/threonine specific protein phosphatase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAGGACCAGGCAGTTGTGTCGGAGCGGCTG |
| Internal bar code: | CATTCCAGAGTGCGTTGAATGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 779 |
| LEAP-Seq percent confirming: | 97.5446 |
| LEAP-Seq n confirming: | 874 |
| LEAP-Seq n nonconfirming: | 22 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCGATTCATAAGCGGTGTTT |
| Suggested primer 2: | TGCAGCAGGATGGTCTGTAG |