Insertion junction: LMJ.RY0402.041471_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.041471
Chromosome:chromosome 12
Location:2895338
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre12.g501950 PPP39 Phosphoprotein phosphatase 2C-related; (1 of 70) 3.1.3.16 - Protein-serine/threonine phosphatase / Serine/threonine specific protein phosphatase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCCCACTCGCCGCCACCCACGTCTCCACCT
Internal bar code:TAAGGGGAGGATAGCTGAATGA

Confirmation - LEAP-Seq

LEAP-Seq distance:545
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:459
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:13

Suggested primers for confirmation by PCR

Suggested primer 1:CCGATTCATAAGCGGTGTTT
Suggested primer 2:TGCAGCAGGATGGTCTGTAG